The conventional triolein absorption test has its defect in that the stool collection was cumbersome, time and energy-wasting. In the present study, the triolein absorption test was carried out using double tracer technique with 125I-triolein and 51Cr2O3 to determine if it can overcome the defect of the conventional method also with satisfactory results. Following were the results:1) The clinical significance of this double tracer method was essentially the same with that previously done by radioactive triolein alone. With the fractional fecal samples, the equation, y=0.626x+2.010 was substantiated, hence, this method appears to be clinically valuable if the appropriate correction is applied. With the mixed fecal samples, the equation y=0.642x+1.468 was substantiated (p<0.005) which appears to be also clinically valuable. When these two data were compared, the equation y=0.975x+0.090 (p<0.05) was substantiated, hence, x≒y. 2) The normal ranges of the fecal triolein excretion rate in this double tracer method were 3.46±1.69%, namely, less than 6.9%. 3) The samplings were done from the first to third defecation in cases of clinically normal, and from the first to second defecation in cases of diarrhea of malabsorption. 4) The intestinal malabsorption of triolein was not observed in whom the triolein absorption was supposed to be clinically normal, however a good number of suspicious malabsorptive cases showed the normal values.