Abstract |
The radioimmunoassay of human thyrotropin was performed
in various thyroid states, utilizing the anti-h-T.S.H.
antibody and purified human thyrotropin supplied from
National Institute of Arthritis and Metabolic Diseases,
Bethesda, Ma., U.S.A., and human thyrotropin standard-A
obtained from National Institute for Biologic
Standards, Mill Hill, London, England. 131I labelled h-
TSH was prepared after the Chloramine-T method of
Greenwood et al. This double antibody system had a
assay sensitivity of about l. 0 μU/ml of plasma HTS-A
and could detect the plasma h-TSH level in the
euthyroid patients. Plasma h-TSH level of the normal 26
Korean was l. 1±0. 83 μU/ml, and that of the 8
hypothyroidisms were 8. 3 to 67. μU/ml. In
hyperthyroidisms, no cases showed the plasma h-TSH
levels over l. 0μU/ ml. Between the hypothyroidism and
euthyroidsm, no overlap is noticed on plasma h-TSH
levels. A case of transient hypothyroid state
identified by determination of plasma h-TSH level is
presented. These results revealed that the
radioimmunoassay of h-TSH in plasma could be a
sensitive method to diagnose the hypothyroidsm, if not
caused by a pituitary disease. |