Cholescintigraphic studies with Tc-99m-HIDA(dimethyl iminodiacetic acid) were performed in 22 cases of normal subjects, 21 of acute cholecystitis, 22 of chronic cholecystitis and 12 others, with the results of, 1) In normal control group, liver and intrahepatic biliary tree, CBD and gall bladder, and G-I tract appeared at 10, 20 and 30 minutes after intravenous injection of Tc-99m-HIDA respectively. 2) In acute cholecystitis, 20 among 21 cases showed non- visualization of gall bladder with the diagnostic accuracy, sensitivity and specificity of 98.290, 95.290 and 100% respectively. 3) In chronic cholecystitis, 5 among 12 cases showed non-visualization of gall bladder and remained 7 among 12 cases showed poor contraction of gall bladder (4), delayed visualization of gall bladder(l) and normal findings(2). 4) In the other disease group, the 12 cases which initially suspected as acute cholecystitis, revealed normal scan findings to exclude the cystic duct obstruction easily. With the above results, this scintigraphic procedure was found very rapid, accurate and easily available method for the determining of the cystic duct patency.