Using chelating agents such as Nitrilotriaceticacid (N'rA), Diethylenetriaminepentaaceticacid (DTPA) and Ethylenediaminenitrilotetraceticacid (EDTA), effects of liposomes labelled with Tc- 99m were determined in vitro and in vivo. Methods of separation and determination of Tc-99m- liposomes added chelating agents were practiced by thin layer chromatogram scan and gel filtration. Biodistributions of Tc-99m- liposomes in normal and sarcoma 180 cells bearing mice were observed. The results were as follows: 1) Maximum amount of Sni+2 to reduction from pertechnetium (10∼20 μci) by adding 0, 1, 10 and 100 μg of SnC12 in 0.2 ml of oxygen free water was 10 μg. 2) The large amounts of SnC12 were not changed but the small amounts of SnC12 were much changed by labeling with Tc-99m to add chelating agents. EDTA in small amounts of SnC12 were reduced more strongly than DTPA or NTA. Using a hydrophilic chelate, DTPA, the uptake of liposomes could not accumulated in liver and spleen but a lipophilic chelate NTA were significant in vivo. 3) UPtake by tumor was achived 1.14% of injected dose per gram tissue and tumor to organ ratios were measured in low with Tc-99m-NTA-liposomes(+).