Abstract |
The ability of 67Ga, administered carrier free as the citrate complex, to localize in human and animal tumors to an extent sufficient to permit visualization of the lesion by scanning is well established. However, neither the mechanism of 67Ga uptake by tumors or inflammatory cells nor its relationship to cell type or to the biochemical status of the cell is yet understood. Author investigated the uptake of 67Ga-citrate using subcellular tissue fractionation of rat livers treated with CCl4 associated with the 3H-thymidine incorporation rate to detect subcellular localization of 67Ga and it's relationship in DNA synthesis. Large amounts of 67Ga associated with the soluble portion of tissue homogenate rather than with isolated cell organelles and not related nuclei residue in the regenerating period after hepatoce lular injury caused by CC14. The elevated uptake of 67Ga in the livers of CC14, treated rats was also inhibited when protein synthesis was stopped by cyclohexamide. Thus protein and the soluble portion of issue homogenates seems to play an important role in the elevated uptake of 67Ga in liver injury induced by CC14 treated rats. |