Abstract |
High affinity complexes for the tumor were obtained by
changing pH and composition in the preparation of Tc-
99m-DMSA. The purpose of this study was to investigate
the tumor affinity, and in vitro and in vivo
characteristics of these complexes. The results
obtained were as follows; 1) Tumor imaging agent was
formed successfully at pH 6.0∼9.0 and renal imaging
agent at pH 2.0∼5.0. 2) The serum protein binding of
Tc-99m-DMSA was 89.1∼92.8% at pH 2.0∼5.0 and
11.8∼30.5% at pH 6.0∼9.0 respectively, and it was not
changed with time. 3) The T 1/2 of tumor affinity
complex in blood between 3 and 6 hours after injection
was 187±29 minutes (mean±SD). 4) In the blood, the
radioactivity was mainly in the plasma, and less than
1% was in the cellular components. 5) In the Walker
carcinosarcoma 256 bearing Wistar rats, the
radioactivity in the kidney increased, and decreased in
the skeleton with time. The radioactivity in the tumor
showed the peak in 6 hours after injection and
decreased thereafter. 6) In the tumor cell, the
radioactivity localized mainly in the cytosol, the
soluble fraction of the cytoplasm. This study provides
the basic knowledge about tumor affinity and usefulness
of Tc-99m-DMSA in the diagnosis of malignant disease. |