Abstract |
For the development of Tc-99m-labelled antimony sulfide
colloid and hydroxyethyl starch, various experiments
such as preparation of colloid, control of the
distribution of particle size, establishment of
labelling conditions, determination of labelling yield
and radiochemical purity, examination of stability, and
organ imagings of rabbits etc. were carried out. 1)
Antimony sulfide colloid was readily prepared by the
reaction of aqueous solution of antimony potassium
tartrate with hydrogen sulfide generated by treating
ferrous sulfide with dilute sulfuric acid. The colloid
could be stabilized by adding small amount of
polyvinylpyrrolidone. 2) Electron microscopy analysis
exhibited the distribution of colloid size in the range
of 1∼15 nm with a major portion of 9 nm. The colloid
solution was sterilized by membrane filtration (0.2
μm) and then stored at 4℃. This sterilized colloid
was so stable that it was usable at least for one year.
3) The antimony sulfide colloid was labelled by adding
sodium pertechnetate-Tc-99m solution to the reaction
vial, followed by adding hydrochloric acid and then
boiled for 30 min. The optimal pH of the reaction
mixture was found to be in the range of 1.3∼1.4.
Instant thin layer chromatography (ITLC) analysis
showed high labelling yield of above 99.5%. This
labelled colloid maintained high radiochemical purity
of above 99% until 10 hours after labelling. 4) Animal
studies showed high uptake of Tc-99m-Sb2S3 colloid at
lymph vessels and nodes indicating a suitable agent for
lymphoscintigraphy. Satisfactory results were also
abtained in other clinical studies. 5) Hydroxyethyl
starch (HES 0.6∼1.0%) was labelled with Na99mTO4, in
the presence of SnCl2 with high labelling yield of
above 99.5%. The optimal pH of the reaction mixture was
in the range of 1.8∼2.0. Tc-99m-HES maintained high
radiochemical purity of above 99% until 10 hours after
labelling. 6) Animal studies showed that Tc-99m-HES
migrated more rapidly from the injection sites into the
lymph vessels than Tc-99m-Sb2S3 colloid while less
amount of the former was uptaken at lymph nodes than
that of the latter. Similar phenomenon was also
observed in other clinical studies. As a result, Tc-
99m-Sb2S3 colloid was found to be more effective
lymphoscintigraphic agent than Tc-99m-HES. |