대한핵의학회지 (1967년~2009년)
대한핵의학회지 1995;29(1)98~7
원저 : 국산 항 백혈병 항체 ( 항 CALLA , 항 JL - 1 ) 의 동위원소 표지 및 면역학적 특성에 관한 연구 ( Radiolabeling and Immunological Characteristics of In - house Anti - leukemic Monoclonal Antibodies ( Anti - CALLA , Anti - JL - 1 Antibodies ) )
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Author 소영(Young So),정준기(June Key Chung),정재민(Jae Min Jeong),이동수(Dong Soo Lee),이명철(Myung Chul Lee),고창순(Chang Soon Koh),
Affiliation
Abstract

Recently murine monoclonal antibodies have studied actively for radioimmuno-scintigraphy and radioinamunotherapy, especially on patients with leukemia and lymphoma. In this research, we studied radiolabeling and immunologic characteristics of two in-house anti-leukemic monoclonal antibodies(anti-CALLA & anti-J1-l antibodies) to make the basis tor their clinical application. Each antibody was radiolabeled successfully with 99mTc by pretargeting transchelation method and with 125I by Iodogen method. We also studied cell binding assay, Scatchard analysis and modulation phenomenon. 125I showed 90% labeling efficiency for each anti-body which was satisfactory, but 99mTc showed labeling efficiency below 70%, for which we need better labeling method, In cell binding assay, the immunoreactivity(IR) was low for 99mTc-labeled antibodies. Scatchard analysis showed satisfactory data for both binding affinity. The affinity constant and antibody binding sites per cell are around 109M-1 and 104, respectively. There was no modulation phenomenon in cases of 125I or 99mTc labeled antibodies. We expect that two anti-leukemic monoclonal antibodies may be useful in diagnosis and therapy for leukemia and lymphoma patients.

Keyword Monoclanal antibody, Radioimmunoscintigraphy, Radioimmunotherapy, Anti-CALLA antibody, Anti-JL-1 antibody
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