원저 : 국산 항 백혈병 항체 ( 항 CALLA , 항 JL - 1 ) 의 동위원소 표지 및 면역학적 특성에 관한 연구 ( Radiolabeling and Immunological Characteristics of In - house Anti - leukemic Monoclonal Antibodies ( Anti - CALLA , Anti - JL - 1 Antibodies ) ) () |
Author |
소영(Young So),정준기(June Key Chung),정재민(Jae Min Jeong),이동수(Dong Soo Lee),이명철(Myung Chul Lee),고창순(Chang Soon Koh), |
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Affiliation |
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Abstract |
Recently murine monoclonal antibodies have studied
actively for radioimmuno-scintigraphy and
radioinamunotherapy, especially on patients with
leukemia and lymphoma. In this research, we studied
radiolabeling and immunologic characteristics of two
in-house anti-leukemic monoclonal antibodies(anti-CALLA
& anti-J1-l antibodies) to make the basis tor their
clinical application. Each antibody was radiolabeled
successfully with 99mTc by pretargeting transchelation
method and with 125I by Iodogen method. We also studied
cell binding assay, Scatchard analysis and modulation
phenomenon. 125I showed 90% labeling efficiency for
each anti-body which was satisfactory, but 99mTc showed
labeling efficiency below 70%, for which we need better
labeling method, In cell binding assay, the
immunoreactivity(IR) was low for 99mTc-labeled
antibodies. Scatchard analysis showed satisfactory data
for both binding affinity. The affinity constant and
antibody binding sites per cell are around 109M-1 and
104, respectively. There was no modulation phenomenon
in cases of 125I or 99mTc labeled antibodies. We expect
that two anti-leukemic monoclonal antibodies may be
useful in diagnosis and therapy for leukemia and
lymphoma patients. |
Keyword |
Monoclanal antibody, Radioimmunoscintigraphy, Radioimmunotherapy, Anti-CALLA antibody, Anti-JL-1 antibody |
Full text Article |
28501422.pdf
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