원저 : 누드마우스에 이식된 인체대장암에서 I - 131 표지 항태아성암항원 단일클론항체를 이용한 방사면역치료법 : 치료성적에 관계되는 인자분석 ( Radioimmunotherapy of Nude Mice Bearing Human Colon Carcinoma with I - 131 Labeled Anti - carcinoembryonic Antigen () |
Author |
김병태(Byung Tae Kim),이경한(Kyung Han Lee),김상은(Sang Eun Kim),최용(Yong Choi),지대윤(Dae Yoon Chi),정준기(June Key Chung),이명철(Myung Chul Lee),고창순(Chang Soon Koh),정홍근(Hong Keun Chung), |
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Affiliation |
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Abstract |
This study was designed to evaluate the effects of
various factors on the therapeutic effect of the I-l3l
labeled anti-carcinoembryonic antigen monoclonal
antibody(anti-CEA antibody). Tetrazolium-based
colorimetric assay (MTT) was used to compare in vitro
cytotoxicity of 3 Korean colon cancer cell lines (SNU-
C2A, SNU-C4, SNU-C5) for selection of proper 2 cell
lines in this study. The changes of the size of tumor
which was xenografted to nude mice (balb/c nu/nu) were
compared in 4 groups (group treated I-131 labeled anti-
CEA antibody, group treated with non-radiolabeled anti-
CEA antibody, group treated with I-131. labeled anti-
human chorionic gonadotropin monoclonal antibody (anti-
hCG antibody) as nonspecific antibody, and group
injected with normal saline as a control).
Immunohistochemical staining and in vivo
autoradiography were performed after excision of the
xenografted tumor. The results were as below mentioned.
The in vitro cytotoxic effect of I-131 labeled anti-CEA
antibody is most prominent in SNU-C5 cell line between
3 cancer cell lines. The changes of xenografted tumor
size in both SNU-C4 and SNU-C5 cell tumors at the
thirteenth day after injection of the antibodies were
smallest in the group treated with I-131 labeled anti-
CEA antibody (SNU-C4/SNU-C5; 324/342%) comparing with
other groups, group treated with anti-CEA antibody
(622/660%), group treated with I-131 anti-hCG antibody
(538/546%), and control group(1030/724%) (p〈0.02 in
SNU-C4 and p〈0.1in SNU-C5 at the 13th day after
injection of antibodies). On the thirteenth day after
injection of the antibodies nude mice were sacreficed
to count the radiouptake of tumor and to check the
changes of tumor size. Correlations between radiouptake
and change of tumor size were calculated in each groups
and significant negative correlation was only obtained
in the group treated with I-131 anti-CEA antibody (p
〈0.05). There were no correlations between antigenic
expression of carcinoembryonic antigen and distribution
of anti-CEA antibody in both SNU-C4 and SNU-C5-cell
tumors on immunoperoxidase staining Qn in vivo
autoradiography the distributions of anti-CEA antibody
were heterogeneous and the intensities of binding were
various in SNU-C4 and SNU-C5 cell tumors. It is
concluded that I-131 labeled tumor-specific monoclonal
antibody, anti-CEA antibody is effective in suppressing
the xenografted tumor growth and the effect is
influenced by sensitivity of tumor cell itself to the
radiolabeled antibody and other local factors instead
of specificity of antibody. |
Keyword |
Radioimmunotherapy, Colon cancer, I-131 labeled anticarcinoembryonic antigen monoclonal antibody |
Full text Article |
28501545.pdf
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