원저 : Iodine - 131 - Iodomethyluridine을 이용한 종양세포증식의 영상화에 관한 실험적 연구 ( Imaging of Tumor Proliferation Using Iodine - 131 - Iodomethyluridine ) () |
Author |
민경윤(Kyung Yoon Min),김창근(Chang Guhn Kim),김현정(Hyun Jeong Kim),임형근(Hyung Guhn Lim),노지영(Ji Young Rho),정선관(Seon Kwan Juhng),원종진(Jong Jin Won),양경문(David J . Yang), |
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Affiliation |
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Abstract |
Purpose: Noninvasive imaging of tumor cell
proliferation could be helpful in the evaluation of
tumor growth potential and could provide an early
assessment of treatment response. Radiolabeled
thymidine, uridine and adenosine have been used to
evaluate tumor cell proliferation. These nucleoside
analogs are incorporated into DNA during proliferation.
Iodine-131-Iodomethyluridine, an analog of Iodine-131-
Iododeoxyuridine, is also involved in DNA/RNA
synthesis. The purpose of this study was to develop
Iodine-131-Iodomethyluridine and image tumor
proliferation using Iodine -131-Iodomethy-luridine.
Materials and Methods: Radiosynthesis of Iodine-131-5-
Iodo-2'-O- methyluridine(Iodine-131-Iodomethyluridine)
was prepared from 10 mg of 2'-O-methyluridine (Sigma
chemical Co., St. Louis, Missouri)-and 2.1 mCi(SP.
10Ci/mg) of Iodine-131-labeled sodium iodide in 100 μl
of water using iodogen reaction. Female Fischer 344
rats were inoculated in the thigh area with breast
tumor cells (13765 NF, 105 cells/rat S.C.). After 14
days, the Iodine-131- Iodomethyluridine 10μCi was
injected to three groups of rats(3/group). The percent
of injected dose per gram of tissue weight was
detennined at 0.5-hours, 2-hours, 4-hours, and 24-hours
respectively. Tumor bearing rats after receiving
Iodine-131-Iodomethyluridine(50μCi/rat, IV) were
euthanized at 2 hours after injection. Autoradiography
was done using freeze-dried 50 μ m coronal section.
After injection of Iodine-131-Iodomethyluridine
(100μCi/rat, IV) in three breast tumor-bearing rats,
planar scintigraphy was taken at 45 minutes, 90 minutes
and 24 hours. Results: Iodine-131-Iodomethyluridine was
conveniently synthesized using iodogen reaction. The
biodistribution showed fast blood clearance and the
tumor-to-tissue uptake ratios showed that optimal
imaging time was at 2 hours postinjection.
Autoradiogram and planar scintigram indicated that
tumor could be well visualized. Conclusion: The
findings suggest that Iodine-131- Iodomethyluridine, a
new radio-iodinated nucleoside, has potential use for
evaluation of active regions of tumor growth. |
Keyword |
Iodine-131-Iodomethyluridine, Tumor Imaging, Tumor Proliferation, Nucleoside, Autoradiography |
Full text Article |
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