대한핵의학회지 (1967년~2009년)
대한핵의학회지 1996;30(3)361~6
원저 : Al3 + 존재가 99mTc - MDP의 표지효율과 생체내 분포에 미치는 영향 ( Effect of Al3 + on Labeling Efficiency and Biodistribution of 99mTc - MDP )
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Author 장영수(Young Soo Chang),정재민(Jae Min Jeong),김영주(Young Ju Kim),곽철은(Cheol Eun Kwark),이동수(Dong Soo Lee),정준기(June Key Chung),이명철(Myung Chul Lee),고창순(Chang Soon Koh),
Affiliation
Abstract

This study was to determine the effect of Al3+ in Tc- 99m eluate from Mo-99-Tc-99m generator on labeling efficiency and biodistribution of Tc-99m-MDP. The chromatographic analysis of Tc-99m-MDP preparations containing Al3+(0-62.5 μg/ml) showed decreased labeling efficiency. Tc-99m pertechnetate and hydrolyzed reduced Tc-99m fraction increased with increasing concentrations of aluminum. However, the chromatography system could not discern between hydrolyzed reduced Tc-99m and Tc-99m labeled colloid. Tc-99m-MDP preparations containing aluminum were relatively stable. Chromatographic analysis also confirmed that no significant differences were observed in the radiochemical purity of the filtered and the unfiltered Tc-99m-MDP preparations containing aluminum by 0.22 μm syringe filter. In biodistribution data of ICR-mice, blood and heart uptake were increasing with increasing concentrations of aluminum, because of decreasing labeling efficiency of Tc-99m-MDP and increasing of Tc-99m pertechnetate. However, liver and bone uptake were not significantly increased. In rat images no difference were observed at 5 μ/mi Al3+ compare with at 10 μg/ml Al3+, but at 10 μg/ml Al3+ lumbar uptake was increased. As a practical conclusion, a concentration below 10 μg/ml Al3+(10 μg/ml Al3+ is the maximum allowed in pertechnetate eluate from Mo-99- Tc-99m generator by USP.) in TC-99m-MDP radiopharmaceutical result in low labeling efficiency Radiochemical purity 90% of Tc-99m-MDP is the minimum allowed by USP. Therefore, when soft tissue uptake is obseved in Tc-99m-MDP bone scan and labeling efficiency is above 90%, we can expect that Al3+ in pertechnetated eluate is not the cause of soft tissue uptake.

Keyword 99mTc-MDP bone scan, Al3 in pertechnetated eluate, soft tissue uptake, labeling efficiency, biodistribution
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