원저 : 항 NCA - 95 단일클론항체의 99mTc 표지 키트 제조 및 특성 연구 ( 99mTc Labeling Kit Preparation and Characteristics of Anti - NCA - 95 Monoclonal Antibody ) () |
Author |
홍미경(Mee Kyoung Hong),정재민(Jae Min Jeong),정준기(June Key Chung),최석례(Seok Rye Choi),김채균(Chae Kyun Kim),이용진(Yong Jin Lee),이동수(Dong Soo Lee),이명철(Myung Chul Lee),고창순(Chang Soon Koh), |
|
Affiliation |
|
Abstract |
The previous monoclonal antibody labeling method for
bone marrow immunoscintigrapy was complicated and
laborious for clinical application. Also it showed a
relatively low labeling efficiency. To improve this
procedure, we compared several direct labeling methods
of Tc-99m. 1) The labeling efficiency in the method
using gluconate as a transchelator was low (40-70%),
but immunoscintigraphy using this radiotracer produced
a clear image. 2) To improve labeling efficiency, β-
mercaptoethanol was removed after reduction. The
labeling efficiency was improved up to 70-80%, but the
radioactivity of the blood pool was high. 3) The higest
labeling efficiency (〉90%) and best quality images
could be obtained by using MDP as a transchelating
agent. It did not require additional procedures for
separation of labeled antibodies. The immunoreactivity
of this antibody was 60%. Residual MDP which can be
taken up by the bone could be removed by PD-10 column.
The reduced antibodies were stable with a high labeling
efficiency (〉90%) for up to 47 days by deep freezing.
We concluded that the improved procedure for Tc-99m
labeling of anti-NCA-95 monoclonal antibody using MDP
as a transchelating agent will be a simple and useful
method for clinical application. |
Keyword |
99mTc-anti NCA-95 monoclonal antibody, Transchelating agent, MDP, Gluconate, Bone marrow immunoscintigraphy |
Full text Article |
28501819.pdf
|
|
|