Abstract |
Cancer tissues are characterized by increased glucose
uptake. 18F-fluorodeoxyglucose(FDG), a glucose analogue
is used for the diagnosis of cancer in PET studies.
This study was aimed to compare the glucose uptake and
glucose transporter l(GLUT1) expression in various
human colon cancer cells. We measured FDG uptake by
cell retention study and expression of GLUTI using
Western blotting. Human colon cancer cells, SNU-C2A,
SNU-C4 and SNU-C5, were used. The cells were incubated
with 1μCi/ml of FDG in HEPES-buffered saline for one
hour. The FDG uptake of SNU-C2A,SNU-C4 and SNU-C5 were
16.8±1.36, 12.3±5.55 and 61.0±2.17cpm/μg of
protein, respectively. Dose-response and time-course
studies represent that FDG uptake of cancer cells were
dose dependent and time dependent. The rate of FDG
uptake of SNU-C2A, SNU-C4 and SNU-C5 were 0.29±0.03,
0.21±0.09 and 1.07±0.07cpm/min/μg of protein,
respectively. Western blot analysis showed that the
GLUT1 expression of SNU-C5 was significantly higher
than those of SNU-C2A and SNU-C4. These results
represent that FDG uptake into human colon cancer cells
are different from each other. In addition, FDG uptake
and expression of CLUT1 are closely related in human
colon cancer cells. |