원저 : Re-188과 Tc-99m 표지 단일클론항체 CEA79.4의 생체외 특성과 생체내 분포 ( In Vitro Properties and Biodistribution of Tc-99m and Re-188 Labeled Monoclonal Antibody CEA79.4 ) () |
Author |
홍미경(Mee Kyoung Hong),정재민(Jae Min Jeong),여정석(Jeong Seok Yeo),김경민(Kyung Min Kim),장영수(Young Soo Chang),이용진(Yong Jin Lee),이동수(Dong Soo Lee),정준기(June Key Chung),이명철(Myung Chul Lee),이승진(Seung Jin Lee), |
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Affiliation |
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Abstract |
Purpose: Radiolabeled CEA79.4 antibody has a
possibility to be used in radioimmunoscintigraphy or
radioimmunotheraphy of cancer. We investigated the in
vitro properties and biodistribution of CEA79.4
antibody labeled with Re-188 or Tc-99m. Materials and
Methods: CEA79.4 was reduced by 2-mercaptoethanol to
produce-SH reside, and was labeled with Re-188 or Tc-
99m. For direct labeling of Tc-99m, methylene-
diphosphonate was used as transchelating agent. CEA79.4
in 50 mM Acetate Buffered Saline (ABS, pH 5.3) was
labeled with Re-188, using stannous tartrate as
reducing agent. In order to measure immunoreactivity
and the affinity constant of radiolabeled antibody,
cell binding assay and Scatchard analysis using human
colon cancer cells SNU-C4, were performed.
Biodistribution study of labeled CEA79.4 was carried
out at 1, 14 and 24 hr in ICR mice. Results:. Labeling
efficiencies of Tc-99m and Re-188 labeled antibodies
were 92.4¡¾5.9% and 84.7¡¾4.6%, respectively. In
vitro stability of Tc-99m-CEA79.4 in human serum was
higher than Re-188-CEA79.4. Immunoreactivity and
affinity constant of Tc-99m-CEA79.4 were 59.2% and
6.59¡¿109 M-1, respectively, while those of Re-188-
CEA79.4 were 41.6% and 4.2¡¿109 M-1, respectively.
After 24 hr of administrations of Re-188 and Tc-99m
labeled antibody, the remaining antibody, the remaining
antibodies in blood were 6.32 and 9.35% ID/g
respectively. The biodistribution of each labeled
antibody in other organs was similar because they did
not accumulate in non-targeted organs. Conclusion: In
vitro properties and biodistribution of Re-188-CEA79.4
were similar to those of Tc-99m-CEA79.4. It appears
that Re-188-CEA79.4 can be used as a suitable agent for
radioimmunotherapy. |
Keyword |
Direct labeling method, Re-188, antibody, Radioimmunotherapy, Generator, Immunoreactivity, Affinity constant |
Full text Article |
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