대한핵의학회지 (1967년~2009년)
대한핵의학회지 1999;33(1)84~10
농양이식백서에서 99mTc , 188Re 직접표지항체의 비교 ( Comparison of Direct-labeling Method of Antibody with 99mTc and 188Re )
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Author 최태현(Tae Hyun Choi),임상무(Sang Moo Lim),최창운(Chang Woon Choi),우광선(Kwang Sun Woo),정위섭(Wee Sup Chung),,임수정(Soo Jeong Lim),
Affiliation
Abstract

Purpose: We investigated the direct labeling method of antibody with 99mTc and 188Re and examined the stability and function of these labeled compounds in in vitro and in vivo. Materials and Methods: Disulfide bond of nonspecific human IgG was reduced to -SH group by 2-mercaptoethanol. Stannous ion was used to reduce 99mTc and 188Re. The stability of 99mTc-IgG and 188Re- IgG was estimated upto 24 hrs. Biodistribution was evaluated in abscess bearing rats at 4 and 24 hr post- injection of 99mTc or 188Re labeled IgG. Results: The number of -SH group per reduced IgG molecule was 2.34. The labeling yield of 99mTc-IgG and 188Re-IgG were 90% and 95%, respectively. The stability of 99mTc-IgG at 1, 4, 6 and 24 hr was 91%, 83%, 78%, 7% and that of 188Re- IgG, high uptake was found on kidney, blood, stomach and abscess (9.42¡¾0.68, 1.43¡¾0.24, 0.86¡¾0.18, 0.72¡¾0.10 %ID/g, respectively). The uptakes at 24 hr were kidney, abscess, stomach, and blood in descending order. In case of 188Re-IgG, high uptake at 4 hr post injection appeared on kidney, blood, abscess and stomach (3.92¡¾0.62, 1.32¡¾0.08, 0.88¡¾0.01, 0.26¡¾0.06, respectively). The upatkes at 24 hr were kidney, abscess, blood abd stomach in descending order. The abscess to blood uptake ratio of 99mTc-IgG was 0.5 at 4 hr and 2.02 at 24 hr and that of 188Re-IgG was 0.67 and 1.29. Conclusion: 99mTc-IgG and 188Re-IgG and 188Re-IgG canbe labeled efficiently with direct labeling method. However, 99mTc-IgG and 188Re-IgG, labeled with direct method, was unstable. Further study in needed to enhance the stability of the antibody labeling.

Keyword IgG, 99mTc-99m, 188Re-188, biodistribution
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