Author |
최태현(Tae Hyun Choi),임상무(Sang Moo Lim),최창운(Chang Woon Choi),우광선(Kwang Sun Woo),정위섭(Wee Sup Chung),,임수정(Soo Jeong Lim), |
Abstract |
Purpose: We investigated the direct labeling method of
antibody with 99mTc and 188Re and examined the
stability and function of these labeled compounds in in
vitro and in vivo. Materials and Methods: Disulfide
bond of nonspecific human IgG was reduced to -SH group
by 2-mercaptoethanol. Stannous ion was used to reduce
99mTc and 188Re. The stability of 99mTc-IgG and 188Re-
IgG was estimated upto 24 hrs. Biodistribution was
evaluated in abscess bearing rats at 4 and 24 hr post-
injection of 99mTc or 188Re labeled IgG. Results: The
number of -SH group per reduced IgG molecule was 2.34.
The labeling yield of 99mTc-IgG and 188Re-IgG were 90%
and 95%, respectively. The stability of 99mTc-IgG at 1,
4, 6 and 24 hr was 91%, 83%, 78%, 7% and that of 188Re-
IgG, high uptake was found on kidney, blood, stomach
and abscess (9.42¡¾0.68, 1.43¡¾0.24, 0.86¡¾0.18,
0.72¡¾0.10 %ID/g, respectively). The uptakes at 24 hr
were kidney, abscess, stomach, and blood in descending
order. In case of 188Re-IgG, high uptake at 4 hr post
injection appeared on kidney, blood, abscess and
stomach (3.92¡¾0.62, 1.32¡¾0.08, 0.88¡¾0.01,
0.26¡¾0.06, respectively). The upatkes at 24 hr were
kidney, abscess, blood abd stomach in descending order.
The abscess to blood uptake ratio of 99mTc-IgG was 0.5
at 4 hr and 2.02 at 24 hr and that of 188Re-IgG was
0.67 and 1.29. Conclusion: 99mTc-IgG and 188Re-IgG and
188Re-IgG canbe labeled efficiently with direct
labeling method. However, 99mTc-IgG and 188Re-IgG,
labeled with direct method, was unstable. Further study
in needed to enhance the stability of the antibody
labeling. |