담체가 Re-188-Hydroxyethylidene Diphosphonate의 표지와 생체내분포에 미치는 영향 ( Effect of Carrier on Labeling and Biodistribution of Re-188-Hydroxyethylidene diphosphonate ) () |
Author |
장영수(Young Soo Chang),정재민(Jae Min Jeong),김보광(Bo Kwang Kim),조정혁(Jung Hyuk Cho),이동수(Dong Soo Lee),정준기(June Key Chung),이승진(Seung Jin Lee),김인걸(In Kul Kim),이상은(Sang Eun Lee),이명철(Myung Chul Lee), |
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Affiliation |
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Abstract |
Purpose: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier (KReO4). Materials and Methods: The kits (HEDP 15 mg, gentisic acid 4 mg and SnCl2.2H2O 4.5 mg) with or without carrier (KReO4 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP. Biodistribution and imaging studies of each preparation were performed in ICR mice (1.85∼3.7 MBq/0.1 ml) and SD rats (74.1∼85.2 MBq/0.5 ml). Results: The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation. Conclusion: The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP. (Korean J Nucl Med 2000;34:344-52) |
Keyword |
Re-188-HEDP, Carrier, Labeling efficiency, Stability |
Full text Article |
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