녹차 폴리페놀이 감마선조사에 의한 백혈병과 림프구모세포의 손상에 미치는 영향의 차이 (Differential Effects of Green Tea Polyphenol in the γ-irradiation Induced Human Leukemic and Lymphoblastic Cell Damage) |
Author |
정환정, 김은미, 민정준, 범희승, 김영호, 정영도, 김창근, |
Hwan-Jeong Jeong, M.D., Eun-Mi Kim, M.S. Jung-Jun Min, M.D., Hee-Seung Bom,M.D., Young-Ho Kim, Ph.D., Young Do Jeong, M.D., Chang Guhn Kim, M.D. |
Affiliation |
원광대학교 의과대학 핵의학교실,전남대학교 의과대학 핵의학교실,조선대학교 자연대학 생물학과,전남대학교 의과대학 미생물학교실 Department of Nuclear Medicine©ö, Research Institute of Clinical Medicine©÷, and Wonkwang Medical Science Research Center©ø, Wonkwang University School of Medicine, Iksan, Korea,Department of Nuclear Medicine4, Chonnam National University School of Medici |
Abstract |
The green tea polyphenol (GTPP) has been known to exert antioxidant activity as a radical
scavenger as well as cancer preventive and cancer growth inhibition effect. The aim of this study was to
identify whether GTPP not only potentiate the growth inhibition effect in γ-irradiated human cancer cell but
also exert protection action for irradiated human normal cell. Materials and Methods: GTPP (80% catechin
including >45% EGCG) added in the HL60, human leukemia, and NC37, human lymphoblast, before
irradiation. After establishing the amount of GTPP and the dose of radiation, the cells were treated with the
GTPP for 6 hours and irradiated with the determined doses. Results: Viability when 10 ㎍/㎖GTPP added
before γ-irradiation with 1 Gy to NC37 cells was not different in comparison with control but it when was
irradiated with 3 Gy significantly different (1 Gy;P=0.126, 3 Gy;P=0.010). 20 ㎍/㎖GTPP did not show
significant difference in both NC37 cells irradiated with 1 Gy and 3 Gy (1 Gy;P=0.946, 3 Gy;P=0.096).
Viabilities were significantly decreased with concentration of additional GTPP in HL60 with 1 or 3 Gy (1 Gy;
69.0±1.7% vs 42.4±1.3%, 3 Gy; 66.9±3.9% vs 44.2±1.6 %). Conclusion: In vitro study, we certified that
when the cells were irradiated with dose below 3 Gy, GTPP provide not only anticancerous effect against
cancer cells but also radioprotective effect in normal cells simultaneously. Theses results suggest the
possibility that consumption of green tea could give the radioprotective effect and maximize the effect on
internal radiation such as radioiodine therapy concomitantly. |
Keyword |
Green tea polyphenol, Radioprotection, radiation, radical scavenger |
Full text Article |
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