대한핵의학회지 (1967년~2009년)
대한핵의학회지 2003;37(6)393~401
마우스 CT26 종양에서 Tc-99m 표지 플루란유도체의 저류 특성
(Retention Characteristics of Tc-99m-Pullulan-Derivatives in CT26 Tumor of Mice)
Author 허영준,송호천,범희승,나건,김성민,
Young-Jun Heo, M.D.,Ho-Chun Song, M.D.,Hee-Seung Bom, M.D.,Kun Na., Ph.D.,Seong Min Kim, M.D.,
Affiliation 전남대학교병원 핵의학과,조선대학교 약학대학,충남대학교병원 핵의학과
Department of Nuclear Medicine, Chonnam National University Hospital, Gwangju, Korea, College of Pharmacy, Chosun University, Department of Nuclear Medicine, Chongnam National University Hospital, Deajeon, Korea
Abstract

Objective: Pullulan derivatives (PD) can be used to make self-assembled hydrogel nanoparticles which are responsive to ionic strength. The aim of this study is to evaluate the potential of PD as a retaining carrier of radioisotope inside tumors. Materials and Methods: Four types of PD were evaluated which included pullulan acetate (PA), succinylated PA (SPA), PA-DTPA and SPA-DTPA conjugates. They were radiolabeled with Tc-99m. Labelling efficiencies were determined at 30 min, 1, 2, 4 and 12 hours after radiolabeling. CT-26 colon cancer cells were subcutaneously injected into Balb/c mice. After 2 weeks of subcutaneous injection, Tc-99m-labelled PD (Tc-99m-PD) were injected into the tumors. Whole body images of mice were obtained at 30 min, 1, 2, and 12 hr after intratumoral injection. All twenty mice were grouped into four groups by largest diameter; control A (largest diameter = 5 mm, n = 5), control B (largest diameter = 10 mm, n = 5), pullulan A (largest diameter = 5 mm, n = 5), pllulan B (largest diameter = 10 mm, n = 5). Dynamic images were obtained for 1 hour after intratumoral injection. Static images were obtained at 1 hr, 2 hr, 3 hr and 4 hr after intratumoral injection with Tc-99m pertechnetate and Tc-99m-PA. Target-to-background ratios and retention rates were calculated. Results: Labeling efficiencies of PA, SPA, PA-DTPA and SPA-DTPA were 94.5 ¡¾ 5.9%, 97.8 ¡¾ 3.5% 94.2 ¡¾ 3.8%, and 92.5 ¡¾ 6.2%, respectively (p>0.05). Percent retention rates (%RR) of PA and PA-DTPA were significantly higher than those of control, however, those of SP-DTPA and SPA became similar to control at 4 and 12 hr, respectively. %RR of pullulan A and pullulan B at 1, 4 and 8 hr is significantly higher than that of control (p < 0.05). However, %RR between pullulan A and pullulan B were similar. Conclusion: The ionic strength dependent PD-nanoparticles are retained in the tumor. No difference of %RR according to tumor size was noted. Therapeutic application of PD labelled with beta- or alpha- emitting radionuclides can be expected.

Keyword Pullulan derivatives; Tc-99m; mouse; CT26; retention.
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