Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 99mTc-Lactosylated Serum Albumin을 이용한 간 기능의 평가 (Evaluation of Liver Function Using 99mTc-Lactosylated Serum Albumin Liver Scintigraphy in Rat with Acute Hepatic Injury Induced by Dimethylnitrosamine) |
Author |
정신영, 이재태, 서명랑, 유정아, 배진호, 안병철, 황재석, 정재민, 하정희, 이규보, |
Shin Young Jeong, Jaetae Lee, Myungrang Seo, Jeong Ah Yoo, Jin Ho Bae, Byeong Cheol Ahn, Jae Seok Hwang, Jae Min Jeong, Jeong-Hee Ha, Kyu Bo Lee |
Affiliation |
경북대학교 의과대학 핵의학교실, 서울대학교 의과대학 핵의학교실, 영남대학교 의과대학 약리학교실 Department of Nuclear Medicine, Kyungpook National University School of Medicine, Seoul National University School of Medicine, Department of Pharmacology, Youngnam University School of Medicine, Daegu, Korea |
Abstract |
Objects: 99mTc-lactosylated human serum albumin (LSA) is a newly synthesized radiopharmaceutical that
binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic
uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by
dimethylnitrosamine (DMN) and results were compared with corresponding findings of liver enzyme profile
and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally
in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after
injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for
30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over
liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual
interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood
clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program.
Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat,
compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat
group than control rat group (DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas
HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control:
0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group.
Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were
decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver
transaminase and degree of histologic liver injury in rat with acute hepatic injury. |
Keyword |
lactosylated human serum albumin, dimethlynitrosamine, liver scintigraphy, asialoglycoprotein |
Full text Article |
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