핵의학 영상을 이용한 chitosan의 galactosylation 효과에 대한 평가 (Nuclear Imaging Evaluation of Galactosylation of Chitosan) |
Author |
정환정1,2,김은미1,2,박인규3,조종수3,김창근1,2,범희승4, |
Hwan-Jeong Jeong, M.D. 1, 2, Eun-Mi Kim, M.S. 1, 2, In-Kyu Park, Ph.D.3, Chong Su Cho, Ph.D.3,Chang-Guhn Kim, M.D. 1, 2, Hee-Seung Bom, M.D.4 |
Affiliation |
원광대학교 의과대학 핵의학교실1, 원광대학교 의과학 연구소2, 서울대학교 농생명공학부3 ,전남대학교 의과대학 핵의학교실4 Department of Nuclear Medicine1, Wonkwang University School of Medicine, Wonkwnag Institute of Medical Science2,Iksan, KoreaSchool of Agricultural Biotechnology3, Seoul National University, Seoul, Korea Department of Nuclear Medicine4, Chonnam National Un |
Abstract |
Purpose: Chitosan has been studied as a non-viral gene delivery vector, drug delivery carrier, metal chelater, food
additive, and radiopharmaceutical, among other things. Recently, galactose-graft chitosan was studied as a
non-viral gene and drug delivery vector to target hepatocytes. The aim of this study was to investigate the
usefulness of nuclear imaging for in vivo evaluation of targeting the hepatocyte by galactose grafting. Methods
and Materials: Galactosyl methylated chitosan (GMC) was produced by methylation to lactobionic acid coupled
chitosan. Cytotoxicity of 99mTc-GMC was determined by MTT assay. Rabbits were injected via their auricular vein
with 99mTc-GMC and 99mTc-methylated chitosan (MC), the latter of which does not contain a galactose group, and
images were acquired with a gamma camera equipped with a parallel hole collimator. The composition of the
galactose group in galactosylated chitosan (GC), as well as the tri-, di-, or mono-methylation of GMC, was
confirmed by NMR spectroscopy. Results: The results of MTT assay indicated that 99mTc-GMC was non-toxic.
99mTc-GMC specifically accumulated in the liver within 10 minutes of injection and maintained high hepatic uptake.
In contrast, 99mTc-MC showed faint liver uptake. 99mTc-GMC scintigraphy of rabbits showed that the galactose
ligand principally targeted the liver while the chitosan functionalities led to excretion through the urinary system.
Conclusion: Bioconjugation with a specific ligand endows some degree of targetability to an administered
molecule or drug, as in the case of galactose for hepatocyte in vivo, and evaluating said targetabililty is a clear
example of the great benefit proffered by nuclear imaging. (Korean J Nucl Med 38(3):253-258, 2004) |
Keyword |
99mTc, hepatocyte-targeted, nuclear imaging, galactosylated chitosan |
Full text Article |
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