갑상선 결절에서 Sodium Iodide Symporter (NIS)의 발현: RT-PCR방법과 면역조직화학염색법의 비교 (Expression of Sodium-Iodide Symporter (NIS) in Thyroid Nodules: Comparison of RT-PCR and Immunohistochemical Staining Methods) |
Author |
배상균, 이강대1, 장희경2, |
Sang Kyun Bae, M.D., Kang Dae Lee, M.D.1, Hee Kyung Chang, M.D.2 |
Affiliation |
인제대학교 의과대학 핵의학교실, 고신대학교 의과대학 이비인후과학교실1, 병리학교실2 Department of Nuclear Medicine, Inje University College of Medicine, Department of Head & Neck Surgery1, Pathology2, Kosin University College of Medicine, Busan, Korea |
Abstract |
Purpose: The sodium-iodide symporter (NIS) expression is an important factor in determining the sensitivity of
radioiodine therapy in well-differentiated thyroid cancers. Several previous studies for the expression of NIS in
thyroid tissues show diverse results. To investigate whether there is difference between methods in determining
the expression of NIS in thyroid tissues of patients with thyroid nodules, we measured the expression of NIS using
two different methods (RT-PCR and immunoshistochemical staining) and compared the results. Materials &
Methods: We measured the expression of NIS by reverse transcriptase-polymerase chain reaction (RT-PCR) and
also by immunohistochemical staining using anti-NIS antibody in thyroid cancers and other benign thyroid
diseases. We compared the results of each method. We included 19 papillary carcinomas, 1 follicular carcinoma, 1
medullary carcinoma, 4 adenomas and 7 nodular hyperplasias. Results: By RT-PCR analysis, 10 of 19 papillary
carcinomas expressed NIS, but 1 follicular cancer didn't express NIS. By immunohistochemical staining, 15 of 19
papaillary carcinomas express NIS, but 1 follicular cancer didn't express NIS. There was a significant correlation
between the semiquantitative results of RT-PCR and immunohistochemical staining of NIS expression. (p<0.01)
Conclusion: Our data demonstrated that the expression of NIS in thyroid cancers and other benign diseases
investigated by RT-PCR and immunohistochemical staining correlated well each other. However, by
immunohistochemical staining, more NIS expression was found.(Korean J Nucl Med 38(6):511-515, 2004) |
Keyword |
Sodium-iodide symporter, RT-PCR, Immunohistochemical stain |
Full text Article |
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