대한핵의학회지 (1967년~2009년)
대한핵의학회지 2005;39(3)200~208
Diethylnitrosamine 및 Thioacetamide 유발 간손상 생쥐에서의 99mTc-Lactosylated Serum Albumin의 체내 분포상
(Biodistribution of 99mTc-Lactosylated Serum Albumin in Mice with Diethylnitrosamine or Thiacetamide Induced Liver Injury)
Author 황재석·안병철·성영옥·서지형·배진호·정신영·유정수·정재민1·이재태·이규보,
Jae Seok Whang, M.D., Byeong Cheol Ahn, M.D., Young Ok Sung, M.D., Ji Hyoung Seo, M.D., Jin Ho Bae, M.D., Shin Young Jeong, M.D., Jung Soo Yoo, M.D., Jae Min Jeong, M.D., Jaetae Lee, M.D., Kyu Bo Lee, M.D.
Affiliation 경북대학교 의과대학 핵의학교실, 서울대학교 의과대학 핵의학교실1
Department of Nuclear Medicine, Kyungpook National University Medical School, Daegu, Korea, Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea
Abstract

Purpose: Tc-99m labeled diethylenetriaminepentaacetic acid (DTPA)-coupled galactosylated human serum albumin (GSA) is a currently used imaging agent for asialoglycoprotein receptor (ASGPR) of the liver, but, it has several shortcomings. Recently a new ASGPR imaging agent, 99mTc-lactosylated human serum albumin (LSA), with simple labeling procedure, high labeling efficiency, high stability was developed. In order to assess the feasibility of the 99mTc-LSA as a ASGPR imaging radiopharmaceuticals, we performed biodistribution study of the tracer in liver injured mice model and the results were compared with histolgic data. Materals and Methods: To induce hepatic damage in ICR mice, diethylnitrosamine (DEN) (60 mg/kg/week X 5 time, low dose or 180 mg/kg/week X 2 times, high dose) and thioacetamide (TAA) (50 mg/kg X 1 time) were administrated intraperitoneally. Degree of liver damage was evaluated by tissue hematoxilin-eosin stain, and expression of asialoglycoprotein receptor (ASGPR) was assessed by immunohistochemistry using ASGPR antibody. 99mTc-LSA was intravenously administrated via tail vein in DEN or TAA treated mice, and biodistribution study of the tracer was also performed. Results: DEN treated mice showed ballooning of hepatocyte and inflammatory cell infiltration in low dose group and severe hapatocyte necrosis in high dose group, and low dose group showed higher ASGPR staining than control mice in immunohistochemical staining. TAA treated mice showed severe hepatic necrosis. 99mTc-LSA Biodistribution study showed that mice with hepatic necrosis induced by high dose DEN or TAA revealed higher blood activity and lower liver activity than control mice, due to slow clearance of the tracer by the liver. The degree of liver uptake was inversely correlated with the degree of histologic liver damage. But low dose DEN treated mice with mild hepatic injury showed normal blood clearance and hepatic activity, partly due to overexpression of ASGPR in mice with mild degree hepatic injury. Conclusion: Liver uptake of 99mTc-LSA was inversely correlated with degree of histologic hepatic injury in DEN and TAA treated mice. These results support that 99mTc-LSA can be used to evaluate the liver status in liver disease patients.(Korean J Nucl Med 39(3):200-208, 2005)

Keyword Asialoglycoprotein receptor, Lactosylated human serum albumin, liver scan, Diethylnitrosamine,
Full text Article 39030200.pdf 39030200.pdf
(03121)서울시 종로구 지봉로 29 금호팔레스빌딩 1705호
TEL : 02-745-2040 FAX : 02-745-3833 E-mail : ksnm@ksnm.or.kr
Copyright 2012 by The Korean Society of Nuclear Medicine(KSNM)