간세포 지향성 Galactosylated Chitosan 화합물의 표지 수율 향상 및 세포 독성에 대한 연구 (A Study on the Labeling Efficiency and Cytotoxicity of Hepatocyte-targeting Galactosylated Chitosan Compounds) |
Author |
김대응1,정환정2,3,김은미2,3,김세림2,3,강윤희2,김민우2,김창근1,손명희2,3, |
Dae-Weung Kim, M.D.1, Hwan-Jeong Jeong, M.D.2,3, Eun-Mi Kim2,3, Se Lim Kim2,3,Yun-Hee Kang, M.D.2, Min-Woo Kim, M.D.2, Chang-Guhn Kim, M.D.1, Myung-Hee Sohn, M.D.2,3 |
Affiliation |
원광대학교 의과대학 핵의학교실1, 전북대학교 의과대학 핵의학교실2, 전북대병원 임상연구소 Department of Nuclear Medicine, Wonkwang University School of Medicine1, Iksan, Department of Nuclear Medicine2,Research Institute of Clinical Medicine3, Chonbuk National University Medical School and the Hospital, Jeonju,Jellabuk-Do, Korea |
Abstract |
Purpose: In prior study, we synthesized 99mTc-
galactosylated chitosan (GC) and performed in vivo
biodistribution
study, showed specific targeting to hepatocyte. The aim
of this study is to evaluate the labeling efficiency
and
cytotoxicity of modified galactosylated chitosan
compounds, galactosyl methylated chitosan (GMC) and
HYNIC-galactosylated chitosan (GCH). Materials and
Methods: GC, GMC and GCH were synthesized and
radiolabeled with 99mTc. Then, they were incubated for
6 hours at room temperature and human serum at 37℃.
Labeling efficiencies were determined at 15, 30 m, 1,
2, 3 and 6 h after radiolabeling. To evaluate
cytotoxicity,
MTT assay was performed in HeLa and HepG2 cells.
Results: In comparison with them of 99mTc-GC, labeling
efficiencies of 99mTc-GMC were significantly improved
(100, 97 and 89% in acetone and 96.3, 95.8 and 75.6% in
saline at 15 m, 1 and 6 h, respectively). Moreover,
99mTc-GCH showed more improved labeling efficiencies
(>95% in
acetone and human serum and >90% in saline at 6 h). In
MTT assay, cytotoxicity was very low and not different
from that of controls. Conclusion: These results
represent that these compounds are radiochemically
compatible
radiopharmaceuticals, can be used in hepatocyte
specific imaging study and in vivo gene or drug
delivery
monitoring.(Korean J Nucl Med 39(5):278-283, 2005) |
Keyword |
99mTc, galactosylated chitosan, hepatocyte, labeling efficiency, cytotoxicity. |
Full text Article |
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