대한핵의학회지 (1967년~2009년)
Nucl Med Mol Imaging 2009;43(4)323~329
핵의학 체외 검사에서 자동분주기를 이용한 Random Assay 가능성평가
(Comparison of Batch Assay and Random Assay Using Automatic Dispenser in Radioimmunoassay)
Author 문승환2, 이호영1, 신선영1, 민경선1, 이현주1, 장수진2, 강지연2, 이동수2, 정준기2, 이명철2,
Seung Hwan Moon, M.D.2, Ho-Young Lee, M.D.1, Sun Young Shin, M.T.1, Gyeong Sun Min, M.T.1, Hyun Joo Lee, M.T.1, Su Jin Jang, M.D.2, ji Yeon Kang, M.D.2, Dong Soo Lee, M.D.2, June Key Chung, M.D.2, and Myung Chul Lee, M.D.2
Affiliation 서울대학교병원운영 서울특별시보라매병원 핵의학과1, 서울대학교 의과대학 핵의학교실2
Department of Nuclear Medicine, 1Seoul Metropolitan Government Seoul National University Boramae Medical Center and 2Seoul National University college of Medicine and, Seoul, Korea
Abstract

Purpose: Radioimmunoassay (RIA) was usually performed by the batch assay. To improve the efficiency of RIA without increase of the cost and time, random assay could be a choice. We investigated the possibility of the random assay using automatic dispenser by assessing the agreement between batch assay and random assay. Materials and Methods: The experiments were performed with four items; Triiodothyronine (T3), free thyroxine (fT4), Prostate specific antigen (PSA), Carcinoembryonic antigen (CEA). In each item, the sera of twenty patients, the standard, and the control samples were used. The measurements were done 4 times with 3 hour time intervals by random assay and batch assay. The coefficient of variation (CV) of the standard samples and patients’ data in T3, fT4, PSA, and CEA were assessed. ICC (Intraclass correlation coefficient) and coefficient of correlation were measured to assessing the agreement between two methods. Results: The CVs (%) of T3, fT4, PSA, and CEA measured by batch assay were 3.2±1.7%, 3.9±2.1%, 7.1±6.2%, 11.2±7.2%. The CVs by random assay were 2.1±1.7%, 4.8±3.1%, 3.6±4.8%, and 7.4±6.2%. The ICC between the batch assay and random assay were 0.9968 (T3), 0.9973 (fT4), 0.9996 (PSA), and 0.9901 (CEA). The coefficient of correlation between the batch assay and random assay were 0.9924(T3), 0.9974 (fT4), 0.9994 (PSA), and 0.9989 (CEA) (p<0.05). Conclusion: The results of random assay showed strong agreement with the batch assay in a day. These results suggest that random assay using automatic dispenser could be used in radioimmunoassay. (Nucl Med Mol Imaging 2009;43(4):323-329)

Keyword Radioimmunoassay, batch assay, random assay, standard curve, coefficient of variation, intraclass correlation coefficient
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