A Sr/Rb generator was prepared by loading Sr to preconditioned tin dioxide column. The generator was eluted by normal saline with flow rate up to 8ml/min, and the eluted radioactivity was monitored by dose calibrator. Radioactivity began to come out at 5ml and reached to peak around 9ml. The total eluted radioactivity increased linearly with flow rate, and the maximum obtained radioactivity was 35mCi at 8ml/min. The Rb preparation was proven to be free from both strontium radioactivity and pyrogen. The Rb was injected to normal female valunteer and positron emission tomographic image of heart was obtained successfully.