Abstract |
In order to develop technetium-99m-labelled human
albumin microsphere (HAM) for lung scintigraphy,
various experiments such as preparation and
fractionation of HAM, establishment of optimal
labelling conditions, determination of radiochemical
purity, stability test and biodistribution of Tc-99m-
HAM were carried out. HAM was prepared from the
suspension of I mi aqueous human serum albumin (25%) in
130 ml of olive oil at 130∼135°with vigorous
stirring. The resulting HAM was fractionated with
microsieve to get the desired particle size (15∼50 μ)
and autoclaved for sterilization. The HAM particles
were treated with stannous chloride and the pH of the
suspension was adjusted to 3.0∼3.5 with phosphate
buffer. After freeze-drying the contents of single
reaction vial containing 5 mg of HAM and 0.2 mg of
SnCl2 it was reacted with Na99mTcO4. The labelling
yield was higher than 99.5% and the stability of Tc-
99m-HAM was high enough to maintain 99.1% of
radiochemical purity up to 24 hours. Lung and liver
uptake in mice was found to be 94% and 0.9%,
respectively. Excellent rabbit and human lung scans
were also obtained. |