원저 : Cyclosporine 방사면역측정법의 정도관리 ( Quality Control for Radioimmunoassay of Cyclosporine ) () |
Author |
정재민(Jae Min Jeong),서일택(Il Tack Seo),문대혁(Dae Hyuk Moon),정준기(June Key Chung),이명철(Myung Chul Lee),조보연(Bo Youn Cho),고창순(Chang Soon Koh), |
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Abstract |
According to the development of monoclonal antibodies
against cyclosporine, it became avaiiable to replace
the conventional polyclonal antibody method with new
monoclonal antibody method to measure the blood leve1
of cyclosporine by radioimrnunoassay. We compared the
results obtained hy the two methods: polyclonal
antibody and monoclonal antibody method. The results
were ohtaincd as follows: 1) We obtained mean value
137,3 ng/ml and CV 16.1% from plasma sample, and mean
values 495.7ng/ml and 1053.8 ng/ml and CVs 19.3% and
17.4% respectively from whole blood sample by
polyclonal antibody method. 2) For the two control
groups, 100 ng/ml 400 ngjml each, we obtained that the
CVs were 20,2% and 14.0% respectively from plasma
sample, and 11.9% and 13.1% respectively from whole
blood samaple by monoclonal antibody method. In
conclusion, we found that ryclosporine RIA was a
relatively reliable method to measure blood or plasma
concentration. Especially RIA using monocloanl antibody
showed less degree of error in measurement compared to
polyclonal antibody method. |
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Full text Article |
28500954.pdf
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