원저 : 인체말초혈액 림프구와 마우스골수세포에서 중기염색체 분석법과 미소핵검사법을 이용한 방사선적응반응 평가 ( Cytogenetic Radiation Adaptive Response Assessed by Metaphase Analysis and Micronuclei Test in Human Lymphocytes and Mouse Bone Marrow Cells )
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| Author |
민정준(Jung Jun Min),범희승(Hee Seung Bom),이승연(Seung Yeon Lee),최근희(Keun Hee Choi),정환정(Hwan Jeong Jeong),송호천(Ho Cheon Song),김지열(Ji Yeul Kim), |
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| Affiliation |
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| Abstract |
Purpose: Radiation adaptive response in human
peripheral lymphocytes and mouse bone marrow cells was
investigated using both metaphase analysis and
micronucleus assay. We assessed the correlation between
both tests. Materials and Methods: Two groups of the
human peripheral lymphocytes and mouse bone marrow
cells were exposed to low dose (conditioning dose, 0.18
Gy) or high dose (challenging dose, 2 Gy) γ-rays. The
other 4 groups were exposed to low dose followed by
high dose after several time intervals (4, 7, 12, and
24 hours, respectively). The frequencies of chromosomal
aberrations in metphase analysis and micronuclei in
micronucleus assay were counted. Results: Chromosomal
aberrations and micronuclei of preexposed group were
lower than those of the group only exposed to high dose
radiation. Maximal reduction in frequencies of
chromosomal aberrations were observed in the group to
which challenging dose was given at 7 hour after a
conditioning dose (p<0.001). Metaphase analysis and
micronucleus assay revealed very good correlation in
both human lymphocytes and mouse bone marrow cells
(r=0.98, p<0.001; r=0.99, p=0.001, respectively).
Conclusion: Radiation adaptive response could be
induced by low dose irradiation in both human
lymphocytes and mouse bone marrow cells. There was a
significant correlation between metaphase analysis and
micronucleus assay |
| Keyword |
Adaptive response, Low level radiation, Human lymphocytes, Mouse marrow cells, Chromosomal aberration, Micronucleus |
| Full text Article |
28502391.pdf 
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