백혈병 세포에서 Multidrug Resistance Gene-1 (mdr1)의 과발현이 99mTc-sestaMIBI 섭취에 미치는 영향 ( Effect of Multidrug Resistance Gene-1 (mdr1) Overexpression on In-Vitro Uptake of 99mTc-sestaMIBI in Murine L1210 Leukemia Cells ) () |
Author |
천경아(Kyung Ah Chun),이재태(Jaetae Lee),이상우(Sang Woo Lee),강도영(Do Young Kang),손상균(Sang Kyun Sohn),이종기(Jong Kee Lee),정준기(June Key Chung),전수한(Soo Han Jun),이규보(Kyu Bo Lee), |
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Affiliation |
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Abstract |
Purpose: To determine whether Tc-99mMIBI is recognized
by the multidrug resistant P-glycoprotein (Pgp), we
have measured quantitatively Tc-99mMIBI uptake in
cancer cells. The effects of various Pgp reversing
agents on cellular Tc-99m-MIBI uptake were also
investigated in the presence of multidrug resistance
gene-1 (mdr1 gene) overexpression. Materials and
Methods: We measured percentage uptake of Tc-99m-MIBI
at different incubation temperatures both in mdr1
positive and negative cells. The effects of verapamil,
cyclosporin, and dipyridamole on cellular uptake of Tc-
99m-MIBI were also evaluated with or without
overexpression of mdr1 gene in cultured murine leukemia
L1210 cells. Results: The mdr1 gene expressing cell
lines were effectively induced in in vitro with
continuous application of low-dose adriamycin or
vincristine. Cellular uptake of Tc-99m-MIBI was higher
in mdr1 negative L1210 cells than those of mdr1
positive cells, and higher when incubated in 37℃ than
4℃. In the presence of verapamil, cyclosporin or
dipyridamole, Tc-99m-MIBI uptake was increased upto
604% in mdr1 positive cells. Conclusion: Cellular
uptake of Tc-99m-MIBI is lower in leukemia cells over-
expressing mdr1 gene, and MDR-reversing agents increase
cellular uptake. These results suggest that Tc-99m-MIBI
can be used for characterizing Pgp expression and
developing MDR-reversing agents In vitro. |
Keyword |
Multidrug resistance, 99mTc-MIBI, MDR-reversing agent |
Full text Article |
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