CT-26 선암을 접종한 마우스에서 Iodine-131-Iodomisonidazole 의 생체분포 및 종양저산소증의 영상화 ( Biodistribution of Iodine-131-Iodomisonidazole and Imaging of Tumor Hypoxia in Mice bearing CT-26 Adenocarcinoma ) () |
Author |
김혜원(Hye-Won Kim),김창근(Chang-Guhn Kim),윤권하(Kwon-Ha Yoon),김현정(Hyun-Jeong Kim),정선관(Seon-Kwan Juhng),노병석(Byung-Suk Roh),(David J Yang),(E Edmund Kim),이현철(Hyun-Chul Lee), |
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Affiliation |
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Abstract |
urpose: Misonidazole is a radiosensitizer that binds in
hypoxic cells. The purpose of this study was to find
out the feasibility of I-131-Iodomisonidazole (IMISO)
for imaging of tumor hypoxia. Materials and Methods:
Tosyl precursor was dissolved in acetonitrile and I-
131-NaI was added to synthesize IMISO. Balb/c mice
inoculated with CT-26 adenocarcinoma were injected with
IMISO. Mice were sacrificed at 1,2,4,24 hr and % of
injected dose per gram of tissue (%ID/g) was
determined. For scintigraphy and MRI, mouse bearing CT-
26 adenocarcinoma was administered with IMISO and
imaging was performed 4 hr after. Then, mouse body was
fixed and microtomized slice was placed on radiographic
film for autoradiography. Results: %ID/g of tumor was
1.64 (1h), 0.98 (2h), 0.85 (4h) and 0.20 (24h),
respectively. At 24h, %ID/g of tumor was higher than
that of all other tissues except thyroid. Tumor to
muscle ratio increased with time and tumor to blood
ratio also increased with time and reached 1.53 at 24
hr. On autoradiogram, tumor was well visualized as an
increased activity in central hypoxic area of the tumor
which corresponds to the area of high signal intensity
on T2-weighted MR image. On scintigraphy, tumor uptake
was visualized. Conclusion: This results suggest that
IMISO may have a potential for tumor hypoxia imaging in
mouse model. However, further study is needed to
improve it's localization in tumor tissue and to
achieve acceptable images of tumor hypoxia. |
Keyword |
Iodine-131-Iodomisonidazole, Tumor hypoxia, Biodistribution, Autoradiography |
Full text Article |
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